Designing of CD8(+) and CD8(+)-overlapped CD4(+) epitope vaccine by targeting late and early proteins of human papillomavirus

Kaliamurthi, Satyavani; Selvaraj, Gurudeeban; Kaushik, Aman Chandra; Gu, Ke-Ren; Wei, Dong-Qing

CD8 + and its overlapped CD4 + epitopes of HPV Notes: a cTl prediction by netcTl 1.2 (threshold >0.75). b Prediction by netMhc ann 3.4; proteasome cleavage score is interpreted as logarithm of the total amount of cleavage site usage liberating the peptide c-terminus; TaP score estimates an effective log(ic 50 ) value for binding to TaP of a peptide or its n-terminal prolonged precursors; Mhc binding prediction score is identical to the class i -log(ic 50 ) values; processing score combines the proteasomal cleavage and TaP transport predictions, and predicts a quantity proportional to the amount of peptide present in the endoplasmic reticulum, where a peptide can bind to multiple Mhc molecules; total score combines the proteasomal cleavage, TaP transport, and Mhc-binding predictions and predicts a quantity proportional to the amount of peptide presented by Mhc molecules on the cell surface. Abbreviations: cTl, cytotoxic T lymphocyte; hla, human leukocyte antigen; hPV, human papillomavirus; Mhc, major histocompatibility complex; TaP, transporter associated with antigen processing.