Author: Berger, Angela K.; Danthi, Pranav
Title: Reovirus Activates a Caspase-Independent Cell Death Pathway Document date: 2013_5_14
ID: jleccqqx_27
Snippet: Quantitation of cell death by AOEB staining. ATCC L929 cells (2 ϫ 10 5 ) grown in 24-well plates were adsorbed with the indicated MOI of reovirus at room temperature for 1 h. The percentage of dead cells after 24, 48, or 72 h incubation was determined using AOEB staining as described previously (30) . For each experiment, Ͼ100 cells were counted, and the percentage of isolated cells exhibiting orange staining (EB positivity) was determined by e.....
Document: Quantitation of cell death by AOEB staining. ATCC L929 cells (2 ϫ 10 5 ) grown in 24-well plates were adsorbed with the indicated MOI of reovirus at room temperature for 1 h. The percentage of dead cells after 24, 48, or 72 h incubation was determined using AOEB staining as described previously (30) . For each experiment, Ͼ100 cells were counted, and the percentage of isolated cells exhibiting orange staining (EB positivity) was determined by epi-illumination fluorescence microscopy using a fluorescein filter set on an Olympus IX71 microscope.
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