Bacterial defiance as a form of prodrug failure

Brandon Alexander Holt; Gabriel A. Kwong

affects key components of the BAH (i.e., r or kcat). Ideally, these variables will proportionally influence either the growth rate or the enzymatic activity of the bacteria, such as how increasing substrate efficiency increases kcat, which creates one critical value (BAHcrit). By contrast, variables that influence growth rate or enzyme activity with multiple critical values (i.e., inflection points) limit the range of applicability. For example, higher temperatures increase both growth rate and 15 enzymatic activity, but above critical temperatures, protein enzymes denature, resulting in irreversible loss of activity 61 . However, these values are beyond relevant biological values and do not limit the applicability of these prodrug treatments to normal physiological conditions.