Author: Brandon Alexander Holt; Gabriel A. Kwong
Title: Bacterial defiance as a form of prodrug failure Document date: 2019_2_21
ID: 9le4s67m_35
Snippet: To estimate bacterial growth rate (r) and enzymatic activity (kcat), we used parameter fitting methods to a simplified model without a prodrug (Fig. S3) . In this model, the prodrug is substituted with a fluorescent probe (P), which is activated with the same substrate sequence as the AMP prodrug. To simplify the model, we defined one bacterium, with ~ 2.5 × 10 3 OmpT proteases per 5 cell 62 , as equivalent to one effective enzyme. The concentra.....
Document: To estimate bacterial growth rate (r) and enzymatic activity (kcat), we used parameter fitting methods to a simplified model without a prodrug (Fig. S3) . In this model, the prodrug is substituted with a fluorescent probe (P), which is activated with the same substrate sequence as the AMP prodrug. To simplify the model, we defined one bacterium, with ~ 2.5 × 10 3 OmpT proteases per 5 cell 62 , as equivalent to one effective enzyme. The concentration of cleaved fluorescent probe is monitored over time, and the bacterial concentration is quantified at serial time points. We fit this data to the two equations modelling bacterial growth and activation of the fluorescent probe (Equations S1.1, S1.2). The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/556951 doi: bioRxiv preprint
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