Author: Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak
Title: BAR scaffolds drive membrane fission by crowding disordered domains Document date: 2018_3_4
ID: drqseaaa_102
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/276147 doi: bioRxiv preprint were quantified from TIRF movies that were taken under identical imaging settings. n = 100, 50, and 91 tubes for N-BAR, Amph-FL, and N-BAR-NfM CTD, respectively. P-values: unpaired, two-tailed Student's t-tests. These data indicate the disordered domains of Amph-FL and N-BAR-NfM CTD did not promote fiss.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/276147 doi: bioRxiv preprint were quantified from TIRF movies that were taken under identical imaging settings. n = 100, 50, and 91 tubes for N-BAR, Amph-FL, and N-BAR-NfM CTD, respectively. P-values: unpaired, two-tailed Student's t-tests. These data indicate the disordered domains of Amph-FL and N-BAR-NfM CTD did not promote fission of lipid tubules in live cells by enhancing protein binding to the membrane surface. Figure S5 . While F-BAR generates membrane tubules, FCHo1-FL forms highly curved fission vesicles. Membrane composition in TEM experiments: 80 mol% DOPC, 5 mol% PtdIns(4,5)P 2 , 15 mol% DOPS. Vesicle composition in (E): 76 mol% DPPC, 15 mol% DPPS, 5 mol% All rights reserved. No reuse allowed without permission.
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