Author: Cho, Sung-Kyu; Kim, Won-Dong
Title: Early diagnosis of radiodermatitis using lactate dehydrogenase isozymes in hairless mice (SKH1-hr) Document date: 2012_12_26
ID: jv1qubm8_13
Snippet: LDH activity assays were performed in 0.1 M potassium phosphate buffer (pH 6.85) containing 1.50 mM pyruvate and 0.14 mM NADH. Changes in absorbency at 340 nm were measured using a spectrophotometer (UV-160A, Shimadzu Co. Ltd., Kyoto, Japan). One unit was defined as the amount of enzyme activity catalyzing the conversion of 1 µmole of substrate per min. Protein content of the extracts was determined according to the method described by Bradford .....
Document: LDH activity assays were performed in 0.1 M potassium phosphate buffer (pH 6.85) containing 1.50 mM pyruvate and 0.14 mM NADH. Changes in absorbency at 340 nm were measured using a spectrophotometer (UV-160A, Shimadzu Co. Ltd., Kyoto, Japan). One unit was defined as the amount of enzyme activity catalyzing the conversion of 1 µmole of substrate per min. Protein content of the extracts was determined according to the method described by Bradford [25] using BSA as the standard.
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