Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells

Lam, Yun W.; Evans, Vanessa C.; Heesom, Kate J.; Lamond, Angus I.; Matthews, David A.

Concluding Remarks-Our data show that adenovirus specifically targets aspects of the nucleolar proteome in contrast to nonspecific disruption by agents like ActD, providing the first high throughput insight into viral-nucleolar interactions. Moreover, by examining the nucleolar proteome, we were able to highlight misdistribution of antigens from all compartments of the cell. We believe this demonstrates the utility of proteomics approaches to generate meaningful data sets that will help us understand the role of the nucleolus in viral infection and to uncover previously unsuspected effects on the host cell during viral infection. Indeed, subcellular fractionation of this sort coupled with confocal microscopy provides a powerful new tool for understanding viral host cell interactions in a broad context and is a positive step toward a systematic understanding of virus host cell interactions.