Author: Zhu, Zhen-Hong; Song, Wen-Qi; Zhang, Chang-Qing; Yin, Ji-Min
Title: Dimethyloxaloylglycine increases bone repair capacity of adipose-derived stem cells in the treatment of osteonecrosis of the femoral head Document date: 2016_9_13
ID: kjvy2c2k_12
Snippet: Four weeks after the surgery, animals were anesthetized with intravenous pentobarbital. The abdominal cavity of the rabbits was opened. A syringe needle was inserted in the abdominal aorta distal to the heart, and the abdominal aorta proximal to the heart was ligated. Then the abdominal vein was cut open, and heparinized normal saline (Shanghai No.1 Biochemical & Pharmaceutical Co., Ltd.) was injected in the vasculature through the needle at a fl.....
Document: Four weeks after the surgery, animals were anesthetized with intravenous pentobarbital. The abdominal cavity of the rabbits was opened. A syringe needle was inserted in the abdominal aorta distal to the heart, and the abdominal aorta proximal to the heart was ligated. Then the abdominal vein was cut open, and heparinized normal saline (Shanghai No.1 Biochemical & Pharmaceutical Co., Ltd.) was injected in the vasculature through the needle at a flow speed of ~20 mm/min. When the outflow was limpid, a silicone injection compound (MICROFIL MV-122; Flow Tech, Inc., Carver, MA, USA) was pumped into the vasculature of the femoral head. The animals were then stored at 4ËšC to ensure polymerization of the contrast agent. After 1 h, the proximal parts of the femurs were harvested and fixed in 4% paraformaldehyde.
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