Author: Lv, Lishan; Li, Xiaoming; Liu, Genmei; Li, Ran; Liu, Qiliang; Shen, Huifang; Wang, Wei; Xue, Chunyi; Cao, Yongchang
Title: Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus Document date: 2014_6_16
ID: k785hl1r_21
Snippet: Murine spleens were collected from sacrificed mice 14 days after the third immunization. Murine splenocytes were obtained using mouse 1× lymphocyte separation medium (Dakewe Biotech, China). The numbers of specific cells that secreted murine IFN-γ (mIFN-γ) or murine IL-4 (mIL-4) were determined by ELISPOT assay (R&D Systems, USA). Splenocytes were stimulated by purified IBV H120 viral particles at a concentration of 10 μg/mL. The number of sp.....
Document: Murine spleens were collected from sacrificed mice 14 days after the third immunization. Murine splenocytes were obtained using mouse 1× lymphocyte separation medium (Dakewe Biotech, China). The numbers of specific cells that secreted murine IFN-γ (mIFN-γ) or murine IL-4 (mIL-4) were determined by ELISPOT assay (R&D Systems, USA). Splenocytes were stimulated by purified IBV H120 viral particles at a concentration of 10 μg/mL. The number of spots was counted by an ImmunoSpot ELISPOT reader (Bioreader4000; BIO-SYS, Germany).
Search related documents:
Co phrase search for related documents- ELISPOT reader and ImmunoSpot ELISPOT reader: 1
- μg 10 concentration and separation medium: 1
- lymphocyte separation medium and mouse lymphocyte separation medium: 1
- lymphocyte separation medium and separation medium: 1, 2
- mouse lymphocyte separation medium and separation medium: 1
- Murine spleen and specific cell: 1
- specific cell and spot number: 1
- specific cell and viral particle: 1, 2, 3, 4, 5, 6, 7, 8, 9
Co phrase search for related documents, hyperlinks ordered by date