Selected article for: "PCR result and sample range"
Author: Zhang, Chunsun; Xing, Da
Title: Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends
  • Document date: 2007_6_18
    • ID: j0bazhy2_18
    Snippet: Small-volume PCR should not only reduce the costs but also allow rapid thermal cycling. However, the use of small-volume PCR does not necessarily reduce amplification time if the heating method does not fully use the advantages of small-volume reaction system (17, 43, 49, 78) . On the contrary, the large-volume PCR can result in the reduction of amplification time when a better mode of heating is utilized (38, 40, 45, 47, 48, 89) . As yet, there .....
    Document: Small-volume PCR should not only reduce the costs but also allow rapid thermal cycling. However, the use of small-volume PCR does not necessarily reduce amplification time if the heating method does not fully use the advantages of small-volume reaction system (17, 43, 49, 78) . On the contrary, the large-volume PCR can result in the reduction of amplification time when a better mode of heating is utilized (38, 40, 45, 47, 48, 89) . As yet, there is no definitive correlation between PCR time and PCR volume. PCR speed ultimately depends on the thermal mass of the entire PCR chip system. Although changeable in a wider range, the sample volume of continuous-flow PCR is usually 510 ml (see Table 1 ).

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