Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage Document date: 2017_9_29
ID: k4gtl2o7_29
Snippet: To summarize, intra-or intermolecular 'stem' structures need to be 5 adjacent to the re-alignment motif for optimal stimulation of base addition. They also need to be 5 adjacent for maximal inhibition of base omission. Taken together, the results show that at the time of productive realignment, the catalytic center of RT is mostly located at the template position 3 adjacent to the 'stem' structure......
Document: To summarize, intra-or intermolecular 'stem' structures need to be 5 adjacent to the re-alignment motif for optimal stimulation of base addition. They also need to be 5 adjacent for maximal inhibition of base omission. Taken together, the results show that at the time of productive realignment, the catalytic center of RT is mostly located at the template position 3 adjacent to the 'stem' structure.
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