Author: Lee, Jong-Han; Chun, Jin-Kyong; Kim, Dong Soo; Park, Yongjung; Choi, Jong Rak; Kim, Hyon-Suk
Title: Identification of Adenovirus, Influenza Virus, Parainfluenza Virus, and Respiratory Syncytial Virus by Two Kinds of Multiplex Polymerase Chain Reaction (PCR) and a Shell Vial Culture in Pediatric Patients with Viral Pneumonia Document date: 2010_9_1
ID: jo29nx9b_20
Snippet: If one or more of the viruses were identified from a multiplex PCR, R-Mix ReadyCell cultures were proceeded with direct immunofluorescence staining. There were 103 samples that showed a positive multiplex PCR of AdV, IFV, PIFV, and RSV. The shell vial culture assay showed 93 positive samples of the total 103 samples (positive rate 90.3%). There were two samples which showed 2 kinds of viruses. A total of 95 positive numbers of the R-Mix culture w.....
Document: If one or more of the viruses were identified from a multiplex PCR, R-Mix ReadyCell cultures were proceeded with direct immunofluorescence staining. There were 103 samples that showed a positive multiplex PCR of AdV, IFV, PIFV, and RSV. The shell vial culture assay showed 93 positive samples of the total 103 samples (positive rate 90.3%). There were two samples which showed 2 kinds of viruses. A total of 95 positive numbers of the R-Mix culture was noted from 15 AdV, 6 IFV A, 9 IFV B, 2 PIFV, and 63 RSV. Positive viral culture rate from the Seeplex TM RV detection kit was 96.1% (99 samples of the total 103 samples) and that of the Labopass TM RV detection kit was 77.7% (80 samples of the total 103 samples).
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