Author: Cobo, Fernando
Title: Application of Molecular Diagnostic Techniques for Viral Testing Document date: 2012_11_30
ID: jiy4cp4n_19
Snippet: PCR allows the synthesis of million of copies of a targeted nucleic acid sequence. This chemical reaction occurs by means of the action of a DNA polymerase that can copy a DNA strand. PCR consists in a mixture of target DNA, two oligonucleotide primers, a DNA polymerase, a mixture of deoxyribonucleotide triphosphates (dNTPs), MgCl 2 , KCl and Tris-HCl buffer. The reaction mixture is heated and cooled during several cycles in a programmable therma.....
Document: PCR allows the synthesis of million of copies of a targeted nucleic acid sequence. This chemical reaction occurs by means of the action of a DNA polymerase that can copy a DNA strand. PCR consists in a mixture of target DNA, two oligonucleotide primers, a DNA polymerase, a mixture of deoxyribonucleotide triphosphates (dNTPs), MgCl 2 , KCl and Tris-HCl buffer. The reaction mixture is heated and cooled during several cycles in a programmable thermal cycler, and after n cycles the target sequence can be amplified 2 n -fold. After the PCR reaction, the detection of product of amplification should be done by means several techniques (e.g. gel analysis, colorimetric detection) [10] .
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