Selected article for: "expression plasmid and transfection reagent"
Author: Watashi, Koichi; Sluder, Ann; Daito, Takuji; Matsunaga, Satoko; Ryo, Akihide; Nagamori, Shushi; Iwamoto, Masashi; Nakajima, Syo; Tsukuda, Senko; Borroto-Esoda, Katyna; Sugiyama, Masaya; Tanaka, Yasuhito; Kanai, Yoshikatsu; Kusuhara, Hiroyuki; Mizokami, Masashi; Wakita, Takaji
Title: Cyclosporin A and its analogs inhibit hepatitis B virus entry into cultured hepatocytes through targeting a membrane transporter, sodium taurocholate cotransporting polypeptide (NTCP)
  • Document date: 2014_4_1
    • ID: ivgwn32n_23
    Snippet: HepG2 cells were transfected with an expression plasmid for NTCP (25) using TransIT LT1 transfection reagent (Mirus). The cells were plated into a 10 cm dish at 4 h posttransfection, and cultured in the presence of G418 1 mg/ml to select plasmid-bearing cells beginning on day 2 posttransfection. After 20 days of growth, individual cell colonies were isolated and expanded. Each cell clone and parental HepG2 cells as a negative control were infecte.....
    Document: HepG2 cells were transfected with an expression plasmid for NTCP (25) using TransIT LT1 transfection reagent (Mirus). The cells were plated into a 10 cm dish at 4 h posttransfection, and cultured in the presence of G418 1 mg/ml to select plasmid-bearing cells beginning on day 2 posttransfection. After 20 days of growth, individual cell colonies were isolated and expanded. Each cell clone and parental HepG2 cells as a negative control were infected with HBV as described above for HepaRG cells, and cell clones highly susceptible to HBV infection were selected for use in experiments.

    Search related documents:
    Co phrase search for related documents
    • cell colony and individual cell colony: 1, 2
    • cell colony and negative control: 1
    • expression plasmid and HBV infection: 1, 2, 3
    • expression plasmid and negative control: 1, 2
    • HBV infection and HepaRG cell: 1, 2
    • LT1 transfection and Mirus LT1 transfection reagent: 1, 2, 3
    • LT1 transfection reagent and Mirus LT1 transfection reagent: 1, 2, 3