Author: Mauthe, Mario; Langereis, Martijn; Jung, Jennifer; Zhou, Xingdong; Jones, Alex; Omta, Wienand; Tooze, Sharon A.; Stork, Björn; Paludan, Søren Riis; Ahola, Tero; Egan, Dave; Behrends, Christian; Mokry, Michal; de Haan, Cornelis; van Kuppeveld, Frank; Reggiori, Fulvio
Title: An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication Document date: 2016_8_29
ID: iuqa0yrw_41
Snippet: with an empty vector or with a plasmid carrying HA-ATG13 (Hieke et al., 2015) , were cultured in DMEM (Thermo Fisher Scientific) supplemented with 100 U/ml penicillin, 100 µg/ml streptomycin, and 10% FCS at 37°C in 5% CO 2 humidified atmosphere. 4 µg/ml blasticidin and 2 µg/ml puromycin were added for culturing the HEK293T-REx cells expressing HA-Flag-ATG13 or HA-Flag-FIP200 (Behrends et al., 2010; Jung et al., 2015) . HeLa-mCeaCam1 and HEK-m.....
Document: with an empty vector or with a plasmid carrying HA-ATG13 (Hieke et al., 2015) , were cultured in DMEM (Thermo Fisher Scientific) supplemented with 100 U/ml penicillin, 100 µg/ml streptomycin, and 10% FCS at 37°C in 5% CO 2 humidified atmosphere. 4 µg/ml blasticidin and 2 µg/ml puromycin were added for culturing the HEK293T-REx cells expressing HA-Flag-ATG13 or HA-Flag-FIP200 (Behrends et al., 2010; Jung et al., 2015) . HeLa-mCeaCam1 and HEK-mCeaCam1 were generated by transducing the cells with mCeaCam1 (murine carcinoembryonic antigen-related cell adhesion molecule 1a) using a Moloney murine leukemia virus packaging vector . Stable cell lines were made using G418 selection. HEK293T-REx, HA-Flag-ATG13, and HA-Flag-FIP200 were generated by retroviral transduction of the according MSCV-i(N-Flag-HA)-IRES-PURO constructs (Behrends et al., 2010; Jung et al., 2015) .
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