Bioinformatics Study on Structural Protein of Severe Acute Respiratory Syndrome Coronavirus 2 (Sars-Cov-2) For Better Understanding the Vaccine Development

Sumaira Gulzar; Saqib Hussain

After this initial process, Surface Protein must be produced with an enzyme known as protease to complete entry into the cell. SARS CoV-2 uses TMPRSS 2 in the same way as SARS-CoV is used Membrane Glycoproteins proteins inside the Golgi system are glycosylated. The modification of the M protein is essential for the virion to attach into the cell and make antigenic protein (de Haan et al., 2003) The protein M plays a key role in the cell's regeneration of virions. N protein forms a complex by binding to genomic RNA and M protein activates the development of interacting virions in this intermediate endoplasmic reticulum-Golgi interface (ERGIC) compartment with this complex (de Haan et al.,1998 ; Escorset al., 2001) Envelop Glycoproteins are tiny proteins consisting of around 76 to 109 amino acids. Approximately 30 amino acids in the E protein N-terminus allow attachment to the virus membrane (Raamsman et al., 2000) Additionally, coronavirus E proteins play a critical role in virion assembly and morphogenesis within the cell. For one research coronavirus E and M proteins were expressed for conjunction with vectors of mammalian expression to form virus-like structures inside the cell (Baudoux et al., 1998; Vennema et al., 1996) In another review, the ability of recombinant mouse hepatitis virus (MHV) and SARS viruses to generate E protein expression in the genome to sustain this status has been significantly reduced (Kuo et al., 2003; DeDiego. et al 2007) N Proteins are helix-capable phosphoproteins with a versatile viral genomic RNA structure. It plays an important role in coronavirus virion assembly, replication and transcription, as the N protein locates both the coronavirus replication / transcription region and the ERGIC region where the virus is collected (Stertzet al., 2007) In this paper, we tried to describe the four structural proteins of SARS-CoV-2 by the use of bioinformatics tools