Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells

Lam, Yun W.; Evans, Vanessa C.; Heesom, Kate J.; Lamond, Angus I.; Matthews, David A.

FIG. 6. Distribution of proteins identified by SILAC as having no change in distribution. All the images are of a fixed focal plane ϳ0.3 m in depth, the DAPI stain is in blue in all cases, and the bar represents 10 m. As before, the top row of images is representative of the location of FLAG-tagged Nop132 fusion protein in Ͼ80% of cells examined. The second row of images shows the location of FLAG-tagged Nop132 in cells infected with adenovirus for 18 h. Viral infection was confirmed by anti-DBP serum. 63 ). As such, it seems reasonable to expect that these factors will be sequestered into regions adjacent to adenovirus DBP because these regions are known to be active in viral mRNA synthesis (50) . Indeed, U2AF 65 , hnRNPU, and SFPQ were all apparently sequestered into a similar pattern that would be consistent with a role for these proteins in viral mRNA metabolism. However, we do not see any particular sequestration into these regions for hnRNPA2/B1, which weakens the case hnRNPA2/B1 plays a role in viral mRNA metabolism, something which can only be determined through further experimentation.