Author: Zhao, Jingxian; Zhao, Jincun; Fett, Craig; Trandem, Kathryn; Fleming, Erica; Perlman, Stanley
Title: IFN-?– and IL-10–expressing virus epitope-specific Foxp3(+) T reg cells in the central nervous system during encephalomyelitis Document date: 2011_8_1
ID: k751ryv4_41
Snippet: MHC class II tetramer staining. CNS-derived mononuclear cells were stained with 8 µg/ml PE-conjugated I-A b /M133 tetramers (obtained from the National Institutes of Health/National Institute of Allergy and Infectious Diseases MHC Tetramer Core Facility, Atlanta, GA) in RP10 media for 1 h at 37°C. Cells were then incubated with anti-CD16/CD32-biotin and anti-CD4-PerCP-Cy5.5, followed by Avidin-APC. Cells were analyzed using a FACSCalibur (BD). .....
Document: MHC class II tetramer staining. CNS-derived mononuclear cells were stained with 8 µg/ml PE-conjugated I-A b /M133 tetramers (obtained from the National Institutes of Health/National Institute of Allergy and Infectious Diseases MHC Tetramer Core Facility, Atlanta, GA) in RP10 media for 1 h at 37°C. Cells were then incubated with anti-CD16/CD32-biotin and anti-CD4-PerCP-Cy5.5, followed by Avidin-APC. Cells were analyzed using a FACSCalibur (BD). Results are shown after gating on CD16/CD32negative cell populations.
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