Author: Conceição-Neto, Nádia; Theuns, Sebastiaan; Cui, Tingting; Zeller, Mark; Yinda, Claude Kwe; Christiaens, Isaura; Heylen, Elisabeth; Van Ranst, Marc; Carpentier, Sebastien; Nauwynck, Hans J.; Matthijnssens, Jelle
Title: Identification of an enterovirus recombinant with a torovirus-like gene insertion during a diarrhea outbreak in fattening pigs Document date: 2017_9_8
ID: kgoczioe_26
Snippet: For peptide identification, raw MS files were converted into mgf.files by Proteome Discover version 1.4 (Thermo Scientific) and processed using Sequest (Eng et al. 1994 ) (HT version 1.3) against a customized in-house built database with sequences from all enterovirus sequences in UniProt and the recombinant enterovirus sequence. A parent mass tolerance of 10 ppm, a fragment tolerance of 0.02 Da, a variable modification oxidation of M, a fixed mo.....
Document: For peptide identification, raw MS files were converted into mgf.files by Proteome Discover version 1.4 (Thermo Scientific) and processed using Sequest (Eng et al. 1994 ) (HT version 1.3) against a customized in-house built database with sequences from all enterovirus sequences in UniProt and the recombinant enterovirus sequence. A parent mass tolerance of 10 ppm, a fragment tolerance of 0.02 Da, a variable modification oxidation of M, a fixed modification with carbamidomethyl C and up to one missed cleavage for trypsin were used. Common MS contaminants, such as human keratin and pig trypsin were used as decoy.
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