Author: Mauthe, Mario; Langereis, Martijn; Jung, Jennifer; Zhou, Xingdong; Jones, Alex; Omta, Wienand; Tooze, Sharon A.; Stork, Björn; Paludan, Søren Riis; Ahola, Tero; Egan, Dave; Behrends, Christian; Mokry, Michal; de Haan, Cornelis; van Kuppeveld, Frank; Reggiori, Fulvio
Title: An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication Document date: 2016_8_29
ID: iuqa0yrw_60
Snippet: After deconvolution, individual siRNAs were used for further experiments (Table S4 ). U2OS cells were transfected for 48 h with 20 nM of control siRNA or of siRNA targeting ATG13, ATG7, ATG101, ULK1, ULK2, or FIP200 (all from GE Healthcare) using 0.1 µl or 2 µl Lipofectamine RNAiMAX (Invitrogen) for 96-or 6-well plate cultures, respectively, according to the manufacturer's protocol. Combinations of two different siRNA probes were performed at t.....
Document: After deconvolution, individual siRNAs were used for further experiments (Table S4 ). U2OS cells were transfected for 48 h with 20 nM of control siRNA or of siRNA targeting ATG13, ATG7, ATG101, ULK1, ULK2, or FIP200 (all from GE Healthcare) using 0.1 µl or 2 µl Lipofectamine RNAiMAX (Invitrogen) for 96-or 6-well plate cultures, respectively, according to the manufacturer's protocol. Combinations of two different siRNA probes were performed at total concentrations of 40 nM and 40 nM of control siRNA were used for these experiments. 10 ng CVB3 and EMCV Renilla luciferase RNA or 2 ng of Cap and IRES (Mengo) Renilla luciferase RNA was transfected into cells in 96well plates using 0.1 µl of RNAiMax.
Search related documents:
Co phrase search for related documents- different combination and total concentration: 1
Co phrase search for related documents, hyperlinks ordered by date