Author: Delli Ponti, Riccardo; Marti, Stefanie; Armaos, Alexandros; Tartaglia, Gian Gaetano
Title: A high-throughput approach to profile RNA structure Document date: 2017_3_17
ID: k23xlzj0_44
Snippet: Since CROSS is fast (less than 2 min to profile a transcript of 20 000 nucleotides), it can be used for highthroughput predictions of the RNA secondary structure. We used CROSS to investigate profiles of sequences taken from CDSs as well as untranslated regions UTRs > 200 000 isoforms (calculated in <72 h) reporting a structural content that is compatible with what is available in literature (1) . We also studied the structural profile of Xist an.....
Document: Since CROSS is fast (less than 2 min to profile a transcript of 20 000 nucleotides), it can be used for highthroughput predictions of the RNA secondary structure. We used CROSS to investigate profiles of sequences taken from CDSs as well as untranslated regions UTRs > 200 000 isoforms (calculated in <72 h) reporting a structural content that is compatible with what is available in literature (1) . We also studied the structural profile of Xist and identified specific regions in agreement with DMS experiments [correlations of 0.63 and 0.75 for Rep E and Rep F (16) ].
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