Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage Document date: 2017_9_29
ID: k4gtl2o7_44
Snippet: The Maize candidate (gi 7262818 71383 R) has the motif 5 -UA4C3-3 . This candidate contains a conserved RNA template forming structure specified by 25 nt 5 to the A4C3 motif (51) , that is a candidate cis-acting RNA roadblocking element for stimulation of RT slippage. LPE analysis showed no relevant slippage for the (sub)-motif AC3 (Supplementary Figure S7B and D, with acyT LPE reaction) but showed marginal slippage-mediated addition of one base .....
Document: The Maize candidate (gi 7262818 71383 R) has the motif 5 -UA4C3-3 . This candidate contains a conserved RNA template forming structure specified by 25 nt 5 to the A4C3 motif (51) , that is a candidate cis-acting RNA roadblocking element for stimulation of RT slippage. LPE analysis showed no relevant slippage for the (sub)-motif AC3 (Supplementary Figure S7B and D, with acyT LPE reaction) but showed marginal slippage-mediated addition of one base under all dNTP condition indicating that the A4 motif in the sequence UA4C3 is a poor but 'active' slippageprone motif (Supplementary Figure S7B and D with acyA LPE reaction).
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