Dimethyloxaloylglycine increases bone repair capacity of adipose-derived stem cells in the treatment of osteonecrosis of the femoral head

Zhu, Zhen-Hong; Song, Wen-Qi; Zhang, Chang-Qing; Yin, Ji-Min

Preparation of transplanted composite. A self-assembling peptide gel (BeaverNano TM hydrogel; Cyagen Biosciences, Inc., Guangzhou, China) was used as the scaffold for loading ASCs. The transplanted composite was prepared by mixing cells with the hydrogels according to the manufacturer's protocol. In brief, 1x10 7 ASCs were suspended in 1 ml 10% sucrose solution (Sinopharm Chemical Reagent Co., Ltd.), and the cell suspension was mixed with the hydrogels in an equal volume. Then, PBS was slowly added to the mixed hydrogel composites in an equal volume and incubated for 30 min at room temperature to allow cross-linking. For the DMOG-treated ASCs, cells were cultured in regular medium with 1,000 µM DMOG for 24 h prior to being mixed with the hydrogel. To ensure ASCs were continuously exposed to DMOG after implantation into the femoral head, 1,000 µM DMOG was added to PBS during the composite mixing procedure.