Blueprint for a Pop-up SARS-CoV-2 Testing Lab

Jennifer A. Doudna

is the (which was not peer-reviewed) The copyright holder for this preprint . Figure 1 . Overview of the Innovative Genomics Institute SARS-CoV-2 Testing Consortium (IGI Consortium) assay and those discussed in this report. A : Overview of Thermo Fisher's TaqPath COVID-19 Multiplex Diagnostic Solution 11, 12 showing the steps as authorized in their EUA (black), and IGI's manual implementation of this workflow (magenta). The IGI's SARS-CoV-2 testing lab was established in two phases. Phase 1 started with automated arraying of patient samples, followed by manual implementation of the Thermo Fisher kit. Phase 2 is a fully automated workflow. B : SARS-CoV-2 genome (NCBI ref NC_045512.2) with translated regions diagrammed in rectangles. Genes targeted by Thermo Fisher's kit are shown in teal 11 , while other open reading frames and gene products are shown in grey. The open reading frame, ORF1ab, targeted by Thermo Fisher's kit encodes non-structural proteins for replication, while the spike (S) and nucleocapsid (N) genes encode two structural proteins. Figure created using BioRender.com.