Selected article for: "RNA polymerase and stem loop structure"
Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage
  • Document date: 2017_9_29
    • ID: k4gtl2o7_7
    Snippet: The starting point for the present work was an unexpected result from a control for experiments in which reverse transcriptase slippage would confound the issue being addressed. A 6bp-stem 4nt-loop nascent transcript structure (here named 'model' stem-loop) stimulates E. coli DNA-dependent RNA polymerase transcriptional realignment at a 3 -A5G5-5 motif which on its own is an inefficient slippage site (47) . Analysis of the product RNA generated i.....
    Document: The starting point for the present work was an unexpected result from a control for experiments in which reverse transcriptase slippage would confound the issue being addressed. A 6bp-stem 4nt-loop nascent transcript structure (here named 'model' stem-loop) stimulates E. coli DNA-dependent RNA polymerase transcriptional realignment at a 3 -A5G5-5 motif which on its own is an inefficient slippage site (47) . Analysis of the product RNA generated in that study involved reverse transcription by SuperScript TM III (derived from Moloney Murine Leukemia Virus RT).

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