Author: Ladner, Jason T.; Beitzel, Brett; Chain, Patrick S. G.; Davenport, Matthew G.; Donaldson, Eric; Frieman, Matthew; Kugelman, Jeffrey; Kuhn, Jens H.; O’Rear, Jules; Sabeti, Pardis C.; Wentworth, David E.; Wiley, Michael R.; Yu, Guo-Yun; Sozhamannan, Shanmuga; Bradburne, Christopher; Palacios, Gustavo
Title: Standards for Sequencing Viral Genomes in the Era of High-Throughput Sequencing Document date: 2014_6_17
ID: kqcx7lrq_10
Snippet: Complete. A genome is complete when the genome sequence has been fully resolved, including all non-protein-coding sequences at the ends of the segment(s). This is typically achieved through rapid amplification of cDNA ends (RACE) or similar procedures. Finished. This final category represents a special instance in which, in addition to having a completed consensus genome sequence, there has been a population-level characterization of genomic dive.....
Document: Complete. A genome is complete when the genome sequence has been fully resolved, including all non-protein-coding sequences at the ends of the segment(s). This is typically achieved through rapid amplification of cDNA ends (RACE) or similar procedures. Finished. This final category represents a special instance in which, in addition to having a completed consensus genome sequence, there has been a population-level characterization of genomic diversity. Typically this requires~400 to 1,000Ï« coverage (see below). This provides the most complete picture of a viral population; however, this designation will apply only for a single stock. Additional characterizations will be necessary for future passages.
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