Author: Lam, Yun W.; Evans, Vanessa C.; Heesom, Kate J.; Lamond, Angus I.; Matthews, David A.
Title: Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells Document date: 2009_10_7
ID: jgxbpy4j_26
Snippet: Confocal Microscopy of Proteins Identified by SILAC as Having No Change in Nucleolar Abundance at 18 h Postinfection-For completeness, we tested a protein that did not change its location at this time point postinfection. Nop132 is a nucleolar antigen that has not been previously investigated in the context of adenovirus infection. We used a FLAGtagged construct to examine its location in uninfected and infected cells. As can be seen from Fig. 6 .....
Document: Confocal Microscopy of Proteins Identified by SILAC as Having No Change in Nucleolar Abundance at 18 h Postinfection-For completeness, we tested a protein that did not change its location at this time point postinfection. Nop132 is a nucleolar antigen that has not been previously investigated in the context of adenovirus infection. We used a FLAGtagged construct to examine its location in uninfected and infected cells. As can be seen from Fig. 6 , Nop132 maintained its apparent nucleolar location with no detectable depletion from the nucleolus to other compartments.
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