Author: Basso, Maria Alexandra; Marques, Cátia; Santos, Marcos; Duarte, Ana; Pissarra, Hugo; Carreira, L Miguel; Gomes, Lídia; Valério-Bolas, Ana; Tavares, Luís; Santos-Gomes, Gabriela; Pereira da Fonseca, Isabel
Title: Successful treatment of feline leishmaniosis using a combination of allopurinol and N-methyl-glucamine antimoniate Document date: 2016_2_10
ID: k9booi08_7
Snippet: Nodular and lymph node aspirates were inoculated in Schneider's Insect Medium (Sigma-Aldrich) supplemented with 0.6% (w/v) of CaCL 2 , 5 mM Hepes, 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin. 18 Cultures were maintained at 24ºC in a refrigerated incubator (Lovibond) and observed by optical microscopy after 8 days of incubation. Viable promastigotes were observed......
Document: Nodular and lymph node aspirates were inoculated in Schneider's Insect Medium (Sigma-Aldrich) supplemented with 0.6% (w/v) of CaCL 2 , 5 mM Hepes, 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin. 18 Cultures were maintained at 24ºC in a refrigerated incubator (Lovibond) and observed by optical microscopy after 8 days of incubation. Viable promastigotes were observed.
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