Author: Zhu, Zhen-Hong; Song, Wen-Qi; Zhang, Chang-Qing; Yin, Ji-Min
Title: Dimethyloxaloylglycine increases bone repair capacity of adipose-derived stem cells in the treatment of osteonecrosis of the femoral head Document date: 2016_9_13
ID: kjvy2c2k_15
Snippet: These samples were then decalcified with 10% ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) at 37˚C for 3 weeks. Following decalcification, the samples underwent micro-CT examination again at a resolution of 18 µm per voxel to evaluate the vascularization of the necrotic area. For segmentation of vessels from background, noise was removed with a low pass Gaussian filter and vessels were defined at the th.....
Document: These samples were then decalcified with 10% ethylenediaminetetraacetic acid (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) at 37˚C for 3 weeks. Following decalcification, the samples underwent micro-CT examination again at a resolution of 18 µm per voxel to evaluate the vascularization of the necrotic area. For segmentation of vessels from background, noise was removed with a low pass Gaussian filter and vessels were defined at the threshold of 85 HU. To reconstruct the three-dimensional architecture of blood vessels in the necrotic area, the vessels were included at each two-dimensional section by built-in 'Contouring Program' for automatic reconstruction of three-dimensional images. The axial slices through the samples were visualized, and the volume of the vessels in the necrotic area was calculated.
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