Author: Zhang, Chunsun; Xing, Da
Title: Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends Document date: 2007_6_18
ID: j0bazhy2_27
Snippet: In this approach, the interior surface of each reaction chamber remains hydrophilic, while the exterior surface is rendered hydrophobic by coating a hydrophobic layer onto the substrate or using a substrate with hydrophobic nature (e.g. silicon) (17, 78) . This differential hydrophilic-hydrophobic coating approach facilitates precise loading and isolated retention of PCR solution in individual chambers, thus avoiding cross contamination between c.....
Document: In this approach, the interior surface of each reaction chamber remains hydrophilic, while the exterior surface is rendered hydrophobic by coating a hydrophobic layer onto the substrate or using a substrate with hydrophobic nature (e.g. silicon) (17, 78) . This differential hydrophilic-hydrophobic coating approach facilitates precise loading and isolated retention of PCR solution in individual chambers, thus avoiding cross contamination between chambers. However, this approach maybe is not robust enough to be of practical usefulness.
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