Author: Gao, Shuibo; Li, Enyao; Gao, Haixia
Title: Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway Document date: 2019_8_21
ID: jwrv3e6j_9
Snippet: The MEG3 expression in HT22 cells was tested by qRT-PCR. Total RNAs were separated by RNAiso Plus (9108, Takara Biomedical Technology, Beijing, China). The cDNA was synthesized with the help of PrimeScript cDNA Synthesis kit (6210, Takara Biomedical Technology). Then, the MEG3 expression was measured by TaqMan Non-coding RNA assay (4426961, Applied Biosystems, Foster City, CA, USA) and compared to b-actin expression. Results were calculated using.....
Document: The MEG3 expression in HT22 cells was tested by qRT-PCR. Total RNAs were separated by RNAiso Plus (9108, Takara Biomedical Technology, Beijing, China). The cDNA was synthesized with the help of PrimeScript cDNA Synthesis kit (6210, Takara Biomedical Technology). Then, the MEG3 expression was measured by TaqMan Non-coding RNA assay (4426961, Applied Biosystems, Foster City, CA, USA) and compared to b-actin expression. Results were calculated using 2 ÀááCt method (Ish-Shalom and Lichter 2010).
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