Author: William Rodriguez; Aman Srivastav; Mandy Muller
Title: C19ORF66 broadly escapes viral-induced endonuclease cleavage and restricts Kaposi’s Sarcoma Associated Herpesvirus (KSHV) Document date: 2018_12_26
ID: 5a4i8v59_33
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/506410 doi: bioRxiv preprint step were performed using in-fusion cloning (Clonetech-takara) and were verified by 315 sequencing. 316 317 RT-qPCR. Total RNA was harvested using Trizol following the manufacture's protocol. cDNAs 318 were synthesized from 1 µg of total RNA using AMV reverse transcriptase (Promega), and used 319 direc.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/506410 doi: bioRxiv preprint step were performed using in-fusion cloning (Clonetech-takara) and were verified by 315 sequencing. 316 317 RT-qPCR. Total RNA was harvested using Trizol following the manufacture's protocol. cDNAs 318 were synthesized from 1 µg of total RNA using AMV reverse transcriptase (Promega), and used 319 directly for quantitative PCR (qPCR) analysis with the SYBR green qPCR kit (Bio-Rad). Signals 320 obtained by qPCR were normalized to 18S. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/506410 doi: bioRxiv preprint assessed using fastqc. Using Galaxy (56), reads were then aligned to the human genome 340 (hg38) by Bowtie2 and differential expression analysis were performed using Cufflink and 341
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