Dieckol, a Component of Ecklonia cava, Suppresses the Production of MDC/CCL22 via Down-Regulating STAT1 Pathway in Interferon-? Stimulated HaCaT Human Keratinocytes

Kang, Na-Jin; Koo, Dong-Hwan; Kang, Gyeoung-Jin; Han, Sang-Chul; Lee, Bang-Won; Koh, Young-Sang; Hyun, Jin-Won; Lee, Nam-Ho; Ko, Mi-Hee; Kang, Hee-Kyoung; Yoo, Eun-Sook

Human adult low-calcium high-temperature (HaCaT) keratinocyte was obtained from the Amore Pacific Company (Gyeonggi-do, Republic of Korea). The cells were cultured in RPMI1640 medium supplemented with 10% FBS and 100 U/ mL penicillin-streptomycin. The cells were maintained at 37 o C in an incubator with a humidified atmosphere of 5% CO 2 . Cell viability was determined using an EZ-cytox-enhanced cell viability assay kit (itsBIO, Korea) following the manufacturer's protocol. Briefly, cells were seeded into the wells of a 96-well plate and treated with IFN-γ (10 ng/mL) in the absence or presence of eckol or dieckol for 24 h. A solution (5 mL) of WST (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2Htetrazolium) was added to each well and incubated for 1 h in an incubator. Then, the absorbance of each well was measured at 450 nm with a VersaMax ELISA microplate reader (Molecular Devices Inc., Sunnyvale, CA, USA).