Selected article for: "nucleic acid Roche isolation kit and Roche isolation kit"
Author: Hernes, S. S.; Quarsten, H.; Hagen, E.; Lyngroth, A. L.; Pripp, A. H.; Bjorvatn, B.; Bakke, P. S.
Title: Swabbing for respiratory viral infections in older patients: a comparison of rayon and nylon flocked swabs
  • Document date: 2010_9_18
    • ID: ivu632j2_21
    Snippet: Extraction of total nucleic acids The rayon swabs were incubated for 15 min in a TRIS-EDTA (TE) buffer. After thorough stirring, 200 μl of each sample was subjected to nucleic acid extraction in a MagNAPure LC instrument (Roche Diagnostics, Mannheim, Germany) using the MagNA Total Nucleic Acid Isolation kit (Roche ca. no. 03038505001). The samples were eluted in 100 μl buffer. To be able to detect inhibition of the PCR assay by specimen-specifi.....
    Document: Extraction of total nucleic acids The rayon swabs were incubated for 15 min in a TRIS-EDTA (TE) buffer. After thorough stirring, 200 μl of each sample was subjected to nucleic acid extraction in a MagNAPure LC instrument (Roche Diagnostics, Mannheim, Germany) using the MagNA Total Nucleic Acid Isolation kit (Roche ca. no. 03038505001). The samples were eluted in 100 μl buffer. To be able to detect inhibition of the PCR assay by specimen-specific inhibitors, a final concentration of 20,000 λ phage DNA copies/ml (TIB MOLBIOL cat. no. 80-5000-02) were added to the TE buffer (not done with the flocked swabs). A control sample with only λ phage DNA was included in all extraction runs, in order to ensure that negative results were not due to poor processing.

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