Author: Hernes, S. S.; Quarsten, H.; Hagen, E.; Lyngroth, A. L.; Pripp, A. H.; Bjorvatn, B.; Bakke, P. S.
Title: Swabbing for respiratory viral infections in older patients: a comparison of rayon and nylon flocked swabs Document date: 2010_9_18
ID: ivu632j2_21
Snippet: Extraction of total nucleic acids The rayon swabs were incubated for 15 min in a TRIS-EDTA (TE) buffer. After thorough stirring, 200 μl of each sample was subjected to nucleic acid extraction in a MagNAPure LC instrument (Roche Diagnostics, Mannheim, Germany) using the MagNA Total Nucleic Acid Isolation kit (Roche ca. no. 03038505001). The samples were eluted in 100 μl buffer. To be able to detect inhibition of the PCR assay by specimen-specifi.....
Document: Extraction of total nucleic acids The rayon swabs were incubated for 15 min in a TRIS-EDTA (TE) buffer. After thorough stirring, 200 μl of each sample was subjected to nucleic acid extraction in a MagNAPure LC instrument (Roche Diagnostics, Mannheim, Germany) using the MagNA Total Nucleic Acid Isolation kit (Roche ca. no. 03038505001). The samples were eluted in 100 μl buffer. To be able to detect inhibition of the PCR assay by specimen-specific inhibitors, a final concentration of 20,000 λ phage DNA copies/ml (TIB MOLBIOL cat. no. 80-5000-02) were added to the TE buffer (not done with the flocked swabs). A control sample with only λ phage DNA was included in all extraction runs, in order to ensure that negative results were not due to poor processing.
Search related documents:
Co phrase search for related documents- MOLBIOL cat and TIB MOLBIOL cat: 1
- negative result and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58
- negative result and nucleic acid extraction: 1, 2, 3, 4
- negative result and PCR assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
- negative result and PCR assay inhibition: 1
- nucleic acid and PCR assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77
- nucleic acid and TE buffer: 1, 2, 3
- nucleic acid and total nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76
- nucleic acid and total nucleic acid extraction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
- nucleic acid and TRIS EDTA TE buffer: 1
- nucleic acid extraction and PCR assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46
- nucleic acid extraction and TE buffer: 1
- nucleic acid extraction and total nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27
- nucleic acid extraction and total nucleic acid extraction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
- PCR assay and TIB MOLBIOL cat: 1
- PCR assay and total nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9
- PCR assay and total nucleic acid extraction: 1, 2, 3, 4
- TE buffer and TRIS EDTA TE buffer: 1, 2, 3, 4
Co phrase search for related documents, hyperlinks ordered by date