Author: Mauthe, Mario; Langereis, Martijn; Jung, Jennifer; Zhou, Xingdong; Jones, Alex; Omta, Wienand; Tooze, Sharon A.; Stork, Björn; Paludan, Søren Riis; Ahola, Tero; Egan, Dave; Behrends, Christian; Mokry, Michal; de Haan, Cornelis; van Kuppeveld, Frank; Reggiori, Fulvio
Title: An siRNA screen for ATG protein depletion reveals the extent of the unconventional functions of the autophagy proteome in virus replication Document date: 2016_8_29
ID: iuqa0yrw_75
Snippet: Figs. S1 and S2 and Table S5 recapitulate the siRNA screen results for every individual virus. Fig. S3 shows the deconvolution experiments of the siRNA probes. Fig. S4 depicts the virus replication experiments upon ATG13 depletion. Fig. S5 and Table S6 resume the results of the MS analysis of FIP200 and ATG13 binding partners in CV-infected and uninfected cells. Fig. S6 shows the immunofluorescence-and EM-based localization studies of EMCV and C.....
Document: Figs. S1 and S2 and Table S5 recapitulate the siRNA screen results for every individual virus. Fig. S3 shows the deconvolution experiments of the siRNA probes. Fig. S4 depicts the virus replication experiments upon ATG13 depletion. Fig. S5 and Table S6 resume the results of the MS analysis of FIP200 and ATG13 binding partners in CV-infected and uninfected cells. Fig. S6 shows the immunofluorescence-and EM-based localization studies of EMCV and CV replication structures. Tables S1 and S2 list the genes that are targeted in the siRNA screen. Table S3 lists the realtime PCR primers that were used in the study. Table S4 lists all the siRNA sequences used in this study. Table S7 collects all the RNA sequencing data. Online supplemental material is available at http ://www .jcb .org /cgi /content /full /jcb .201602046 /DC1.
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