Author: Fisher, Cynthia; Bornstein, Rebecca; Kuypers, Jane; Boeckh, Michael; Limaye, Ajit; Jerome, Keith
Title: A Prospective Study Comparing Self-Collected Nasal Swabs to Oral Washes for Monitoring Viral Load Kinetics in Lung Transplant Recipients With Respiratory Virus Infection Document date: 2016_10_24
ID: kgkyh0w5_2
Snippet: Methods. LTR with clinically diagnosed, laboratory-confirmed RVI were eligible. After informed consent, research coordinators demonstrated collection technique and provided written instructions. Patients self-collected daily paired nasal swabs and oral washes for 14 days after initial RVI diagnosis, and samples were given directly to coordinators (inpatients) or mailed to the laboratory (outpatients). Samples were tested using a validated, publis.....
Document: Methods. LTR with clinically diagnosed, laboratory-confirmed RVI were eligible. After informed consent, research coordinators demonstrated collection technique and provided written instructions. Patients self-collected daily paired nasal swabs and oral washes for 14 days after initial RVI diagnosis, and samples were given directly to coordinators (inpatients) or mailed to the laboratory (outpatients). Samples were tested using a validated, published laboratory-developed real-time PCR assay. Samples with cycle threshold (Ct) values >40 were considered negative. We used descriptive statistics and patient reports of acceptability to compare the two methods.
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