Selected article for: "cleavage site and native processing site"

Title: The propeptide of preprosomatostatin mediates intracellular transport and secretion of alpha-globin from mammalian cells
  • Document date: 1989_5_1
  • ID: kjid2e3q_47
    Snippet: PRO-GLO was accurately processed indicating that the native propeptide cleavage site (ArgLys) was recognized by the paired basic processing enzyme(s). Thus, even though chimpanzee ct-globin possesses a single pair of basic amino acids (Lys~ and Lys~:), most probably these were not cleaved since no smaller forms of globin-related peptides were detected. Accurate processing demonstrated the conformational dominance of the propeptide and in particul.....
    Document: PRO-GLO was accurately processed indicating that the native propeptide cleavage site (ArgLys) was recognized by the paired basic processing enzyme(s). Thus, even though chimpanzee ct-globin possesses a single pair of basic amino acids (Lys~ and Lys~:), most probably these were not cleaved since no smaller forms of globin-related peptides were detected. Accurate processing demonstrated the conformational dominance of the propeptide and in particular the highly conserved hexapeptide region. This is noteworthy since a-globin is 10 times the size of mature SRIF and nearly twice the size of the proregion itself; thus, it is unlikely that the chimera and native precursor would be structurally related.

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