Author: Lv, Lishan; Li, Xiaoming; Liu, Genmei; Li, Ran; Liu, Qiliang; Shen, Huifang; Wang, Wei; Xue, Chunyi; Cao, Yongchang
Title: Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus Document date: 2014_6_16
ID: k785hl1r_11
Snippet: Sf9 cells were grown on 24 × 24 mm glass coverslips in 6-well culture plates and infected with both rBV-NA/S1 and rBV-M1 at a MOI of 5 for 48 h and then fixed. The rBV-M1 infected cells were treated with 0.3% TrintonX-100, while control cells were infected with wild-type baculoviruses and processed in parallel with other samples. The expression of fusion protein NA/S1 was detected by chicken polyclonal sera against IBV H120 virus and FITC-conjug.....
Document: Sf9 cells were grown on 24 × 24 mm glass coverslips in 6-well culture plates and infected with both rBV-NA/S1 and rBV-M1 at a MOI of 5 for 48 h and then fixed. The rBV-M1 infected cells were treated with 0.3% TrintonX-100, while control cells were infected with wild-type baculoviruses and processed in parallel with other samples. The expression of fusion protein NA/S1 was detected by chicken polyclonal sera against IBV H120 virus and FITC-conjugated donkey anti-chicken secondary antibody (FITC, green; Beijing Biosynthesis Biotechnology, China). M1 protein was detected by mouse polyclonal sera against influenza virus H5N1 and CY3-conjugated rabbit anti-mouse secondary antibody (CY3, red; Beijing Biosynthesis Biotechnology). The nucleus was stained with DAPI (Beijing Biosynthesis Biotechnology). Coverslips were mounted on glass slides and analyzed on a Leica TCS-SP5 confocal laser scanning microscope.
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