Author: Xin Zeng; Lingfang Li; Jing Lin; Xinlei Li; Bin Liu; Yang Kong; Shunze Zeng; Jianhua Du; Huahong Xiao; Tao Zhang; Shelin Zhang; Jianghai Liu
Title: Blocking antibodies against SARS-CoV-2 RBD isolated from a phage display antibody library using a competitive biopanning strategy Document date: 2020_4_20
ID: 4w6caxwu_7
Snippet: A synthetic human Fab antibody library AB1 (LibAB1) was constructed according to a procedure previously described [7] . Human germline immunoglobulin variable segments VH3-30 and VL1-16 were employed as templates, the complementarity-determining regions L3 (CDR-L3) and H3 (CDR-H3) was diversified by the designed mutagenic oligonucleotides. The oligonucleotides were synthesized using the trimer phosphoramidites mix Z (Glen Research) containing cod.....
Document: A synthetic human Fab antibody library AB1 (LibAB1) was constructed according to a procedure previously described [7] . Human germline immunoglobulin variable segments VH3-30 and VL1-16 were employed as templates, the complementarity-determining regions L3 (CDR-L3) and H3 (CDR-H3) was diversified by the designed mutagenic oligonucleotides. The oligonucleotides were synthesized using the trimer phosphoramidites mix Z (Glen Research) containing codons for 12 amino acids in the following molar ratios: 20% each Y, S &G, 6% each T & A, and 4% each P, H, R, F, W, V & L. The number of positions denoted by Z in CDR-L3 (QQ (Z)n PLT) and -H3 (AR (Z) n (A/G/D/Y) FDY) was varied from 3 to 12 and 8 to 12, respectively. The library size is estimated to be 1×10 12 .
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