Author: Penno, Christophe; Kumari, Romika; Baranov, Pavel V.; van Sinderen, Douwe; Atkins, John F.
Title: Stimulation of reverse transcriptase generated cDNAs with specific indels by template RNA structure: retrotransposon, dNTP balance, RT-reagent usage Document date: 2017_9_29
ID: k4gtl2o7_31
Snippet: To explore potentially relevant properties of G-rich sequences, four dsDNA constructs were made (Supplementary Methods). RNA generated from these with T7 RNA polymerase had the sequence GGCGGCGGCGG 5 adjacent to the A7 motif or separated from it by 1, 2 or 3 nt (C, UC or UUC) ( Figure 3A ). In the 5 leader (UTR) of eukaryotic initiation factor-4A (eIF4A) mRNA this sequence forms an RNA quadruplex (50) . However, the structure potentially formed i.....
Document: To explore potentially relevant properties of G-rich sequences, four dsDNA constructs were made (Supplementary Methods). RNA generated from these with T7 RNA polymerase had the sequence GGCGGCGGCGG 5 adjacent to the A7 motif or separated from it by 1, 2 or 3 nt (C, UC or UUC) ( Figure 3A ). In the 5 leader (UTR) of eukaryotic initiation factor-4A (eIF4A) mRNA this sequence forms an RNA quadruplex (50) . However, the structure potentially formed in the transcripts utilized here could be different due the potential for pairing involving the U and C, where present, in the spacer, and was not explored.
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