Selected article for: "internal control and Master mix"
Author: Zhou, Ping; Zhai, Shanli; Zhou, Xiang; Lin, Ping; Jiang, Tengfei; Hu, Xueying; Jiang, Yunbo; Wu, Bin; Zhang, Qingde; Xu, Xuewen; Li, Jin-ping; Liu, Bang
Title: Molecular Characterization of Transcriptome-wide Interactions between Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Porcine Alveolar Macrophages in vivo
  • Document date: 2011_8_7
    • ID: js7l86fh_15
    Snippet: The RNA samples prepared for microarray analysis were also used for Q-PCR verification. Reverse transcriptions were performed using M-MLV Reverse Transcriptase (Promega) according to the manufacturer's instructions. The primers were designed with the Primer Premier 5.0 program. The RPL32 gene was used as the internal control [20] . The primer sequences, melting temperatures and product sizes are shown in Table 1 . Q-PCR was performed on the Light.....
    Document: The RNA samples prepared for microarray analysis were also used for Q-PCR verification. Reverse transcriptions were performed using M-MLV Reverse Transcriptase (Promega) according to the manufacturer's instructions. The primers were designed with the Primer Premier 5.0 program. The RPL32 gene was used as the internal control [20] . The primer sequences, melting temperatures and product sizes are shown in Table 1 . Q-PCR was performed on the LightCycler 480Ⅱ (Roche, Basel, Sweden) using SYBR Green Realtime PCR Master Mix (TOYOBO CO., LTD, Japan) as the readout. Data was analyzed by the 2 -ΔΔCT method [21] . The data analysis procedure was performed as described previously [15] .

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