Selected article for: "image plus Pro and MD software"
Author: Zhu, Zhen-Hong; Song, Wen-Qi; Zhang, Chang-Qing; Yin, Ji-Min
Title: Dimethyloxaloylglycine increases bone repair capacity of adipose-derived stem cells in the treatment of osteonecrosis of the femoral head
  • Document date: 2016_9_13
    • ID: kjvy2c2k_16
    Snippet: Histology and immunohistochemistry. After micro-CT scanning, samples were dehydrated and made transparent using dimethylbenzene (Sinopharm Chemical Reagent Co., Ltd.). They were then embedded in wax and sectioned into 6 µm coronal planes. Sections were stained with hematoxylin and eosin (H&E) and observed under a light microscope (Leica Microsystems GmbH, Wetzlar, Germany). Image-Pro Plus 6.0 software (Media Cybernetics, Inc., Rockville,MD, USA).....
    Document: Histology and immunohistochemistry. After micro-CT scanning, samples were dehydrated and made transparent using dimethylbenzene (Sinopharm Chemical Reagent Co., Ltd.). They were then embedded in wax and sectioned into 6 µm coronal planes. Sections were stained with hematoxylin and eosin (H&E) and observed under a light microscope (Leica Microsystems GmbH, Wetzlar, Germany). Image-Pro Plus 6.0 software (Media Cybernetics, Inc., Rockville,MD, USA) was used to evaluate new bone formation at x100 magnification in six randomly selected fields per section. Bone density was defined as the ratio of new bone area to total area. The border of the new bone and osteoid tissue was difficult to define, so osteoid tissue was not included in new bone calculations.

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