Author: Lv, Lishan; Li, Xiaoming; Liu, Genmei; Li, Ran; Liu, Qiliang; Shen, Huifang; Wang, Wei; Xue, Chunyi; Cao, Yongchang
Title: Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus Document date: 2014_6_16
ID: k785hl1r_28
Snippet: protein from H5N1 (Fig. 1A) . The estimated molecular weight (MW) was about 100 KD for NA/S1 fusion protein and 25 KD for M1 protein. Western blot analysis showed that NA/S1 was detected as a 100 KD band (Fig. 1B) , while M1 protein appeared as a 25 KD band (Fig. 1C) . It should be noted that one faint 100 KD band was detected together with M1 protein when probed with chicken polyclonal antibodies against whole influenza H5N1 virus, suggesting th.....
Document: protein from H5N1 (Fig. 1A) . The estimated molecular weight (MW) was about 100 KD for NA/S1 fusion protein and 25 KD for M1 protein. Western blot analysis showed that NA/S1 was detected as a 100 KD band (Fig. 1B) , while M1 protein appeared as a 25 KD band (Fig. 1C) . It should be noted that one faint 100 KD band was detected together with M1 protein when probed with chicken polyclonal antibodies against whole influenza H5N1 virus, suggesting that the chicken polyclonal antibodies could react with the CT and TM domains of NA protein. Nevertheless, these results demonstrated that NA/S1 and M1 were properly expressed in Sf9 cells.
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