Long non-coding RNA MEG3 attends to morphine-mediated autophagy of HT22 cells through modulating ERK pathway

Gao, Shuibo; Li, Enyao; Gao, Haixia

Further experiments were carried out to investigate whether MEG3 took part in the morphine-caused elevations of OX1R, cfos, p/t-ERK and p/t-PKC expression in HT22 cells. Data in Figure 5 (A) presented that there was no significant difference of OX1R protein level in morphine þ si-NC group and morphine þ si-MEG3 group. Figure 5 (B) displayed that morphine-caused elevation of c-fos expression was notably weakened by si-MEG3 transfection (p ˂ 0.05). Besides, morphine-caused enhancement of p/t-ERK expression in HT22 cells was remarkably mitigated by si-MEG3 transfection ( Figure 5(C) , p ˂ 0.05), while the p/t-PKC expression in HT22 cells was not changed between transfection with si-NC and transfection with si-MEG3. These outcomes illustrated that up-regulation of MEG3 also took part in the influences of morphine on c-fos and p/t-ERK expressions in HT22 cells.