C19ORF66 broadly escapes viral-induced endonuclease cleavage and restricts Kaposi’s Sarcoma Associated Herpesvirus (KSHV)

William Rodriguez; Aman Srivastav; Mandy Muller

HiSeq4000. Resulting reads were aligned to the human genome (hg38) using Bowtie, replicates 100 were merged using CuffCompare and significant expression fold change between mock and 101 each of the endonuclease conditions were assessed by CuffDiff (Figure 1, Figure S1 & Table 102 S1). The reproducibility between replicate experiments was high ( Fig. 1A-D) , which is in line 103 with previous reports showing that these endonucleases target transcripts in a 104 selective/sequence-specific manner as previously observed (13). As expected, a number of 105 transcripts were significantly affected upon expression of the various herpesviral endonucleases 106 ( Fig. S1) : we observed that between 55-60% of total mRNA were degraded, with muSOX being 107 the most effective of the endonucleases tested here (Fig. S1 ). This rate of degradation is within 108 range of what was observed before (16). Gene Ontology (GO) analysis on the transcripts 109 spared from degradation revealed that they encode proteins that have a wide array of functions, 110 ranging from ion binding to RNA binding (Fig S2) . 111 All rights reserved. No reuse allowed without permission.