Author: Cobo, Fernando
Title: Application of Molecular Diagnostic Techniques for Viral Testing Document date: 2012_11_30
ID: jiy4cp4n_55
Snippet: HDA is an isothermal amplification method similar to DNA replication in vivo by using a DNA helicase to separate two complementary DNA strands (dsDNA) and further extension by a DNA polymerase. The initial heat denaturation and subsequent thermocycling are not necessary, and the entire HDA reaction can be performed at a single uniform temperature. This technique provides a useful tool to amplify DNA in vitro under isothermal conditions. HDA techn.....
Document: HDA is an isothermal amplification method similar to DNA replication in vivo by using a DNA helicase to separate two complementary DNA strands (dsDNA) and further extension by a DNA polymerase. The initial heat denaturation and subsequent thermocycling are not necessary, and the entire HDA reaction can be performed at a single uniform temperature. This technique provides a useful tool to amplify DNA in vitro under isothermal conditions. HDA technique has been developed to detect several viruses in different clinical samples such as HIV-1 in human plasma [43] and HSV types 1 and 2 from genital lesions [44] .
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