Designing of CD8(+) and CD8(+)-overlapped CD4(+) epitope vaccine by targeting late and early proteins of human papillomavirus

Kaliamurthi, Satyavani; Selvaraj, Gurudeeban; Kaushik, Aman Chandra; Gu, Ke-Ren; Wei, Dong-Qing

VaxiJen is the first server developed for the prediction of antigenicity of proteins of viruses, bacteria, fungi, parasites, and tumor cells. 45 The potential antigen classification is completely based on the physicochemical properties of proteins and not related to sequence alignment. Therefore, we utilized VaxiJen tool in the present study and predicted potential antigenic proteins with 87% accuracy. The reliable predictions of CTL epitopes are vital for consistent drug development. 82, 83 The CD8 + receptors consist of single CD8α and CD8β chains. Furthermore, the CD8 + CTLs identify the epitopes which are presented by the MHC class I established on all the nucleated cells. This process is facilitated by the binding of CD8 + to α3 conserved region of MHC class I. Recently, elution studies by Bassani-Sternberg et al 84 proved that the 9mer peptides are naturally optimized by the antigen processing process. This result supports the present study.