Author: Lee, Jong-Han; Chun, Jin-Kyong; Kim, Dong Soo; Park, Yongjung; Choi, Jong Rak; Kim, Hyon-Suk
Title: Identification of Adenovirus, Influenza Virus, Parainfluenza Virus, and Respiratory Syncytial Virus by Two Kinds of Multiplex Polymerase Chain Reaction (PCR) and a Shell Vial Culture in Pediatric Patients with Viral Pneumonia Document date: 2010_9_1
ID: jo29nx9b_15
Snippet: Ten samples, which were culture negative but multiplex PCR positive, were proceeded with sequencing analysis to identify these equivocal results. All sequencing analyses were proceeded after repeating PCR then proper primers were prepared for specific viruses identification. An automated sequencing analyzer (Applied Biosystems, Foster City, CA, USA) accompanied with its recommended reagent was used according to the manufacturer's protocol. Finall.....
Document: Ten samples, which were culture negative but multiplex PCR positive, were proceeded with sequencing analysis to identify these equivocal results. All sequencing analyses were proceeded after repeating PCR then proper primers were prepared for specific viruses identification. An automated sequencing analyzer (Applied Biosystems, Foster City, CA, USA) accompanied with its recommended reagent was used according to the manufacturer's protocol. Finally, results of all sequences were analyzed by matching those of GenBank data using Basic Local Alignment Search Tool (BLAST) (http://blast.ncbi.nlm.nih.gov/Blast.cgi).
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