Selected article for: "blast search and PCR analysis"
Author: Lee, Jong-Han; Chun, Jin-Kyong; Kim, Dong Soo; Park, Yongjung; Choi, Jong Rak; Kim, Hyon-Suk
Title: Identification of Adenovirus, Influenza Virus, Parainfluenza Virus, and Respiratory Syncytial Virus by Two Kinds of Multiplex Polymerase Chain Reaction (PCR) and a Shell Vial Culture in Pediatric Patients with Viral Pneumonia
  • Document date: 2010_9_1
    • ID: jo29nx9b_15
    Snippet: Ten samples, which were culture negative but multiplex PCR positive, were proceeded with sequencing analysis to identify these equivocal results. All sequencing analyses were proceeded after repeating PCR then proper primers were prepared for specific viruses identification. An automated sequencing analyzer (Applied Biosystems, Foster City, CA, USA) accompanied with its recommended reagent was used according to the manufacturer's protocol. Finall.....
    Document: Ten samples, which were culture negative but multiplex PCR positive, were proceeded with sequencing analysis to identify these equivocal results. All sequencing analyses were proceeded after repeating PCR then proper primers were prepared for specific viruses identification. An automated sequencing analyzer (Applied Biosystems, Foster City, CA, USA) accompanied with its recommended reagent was used according to the manufacturer's protocol. Finally, results of all sequences were analyzed by matching those of GenBank data using Basic Local Alignment Search Tool (BLAST) (http://blast.ncbi.nlm.nih.gov/Blast.cgi).

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