Molecular Characterization of Transcriptome-wide Interactions between Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus and Porcine Alveolar Macrophages in vivo

Zhou, Ping; Zhai, Shanli; Zhou, Xiang; Lin, Ping; Jiang, Tengfei; Hu, Xueying; Jiang, Yunbo; Wu, Bin; Zhang, Qingde; Xu, Xuewen; Li, Jin-ping; Liu, Bang

After infection with PRRSV-WUH3, the piglets presented typical clinical signs, e.g. fever, asthma, coughing, anorexia, lethargy and convulsion. The average rectal temperature rose to above 40.5 ºC at 2 dpi and seemed to peak at 5 dpi. The two piglets surviving at 5 dpi showed a slight decrease of rectal temperature in the following two days ( Figure 1A ). To assess the replication and spread of HP-PRRSV, the viral copy number/ml in serum was determined by absolute real-time quantitative-PCR ( Figure 1B ). The level of viremia increased rapidly during the first two days post-infection, then increased slowly from 3 to 5 dpi, and approached the plateau phase at 6 or 7 dpi.